Sequential resonance assignment
نویسندگان
چکیده
A 500-MHz ‘H-NMR study on a double-stranded non-self-complementary DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in thegal operon is presented. Using pre-steadystate nuclear Overhauser effect (NOE) measurements, all exchangeable imino, non-exchangeable base, methyl, and HI’, H2’ and H2” sugar proton resonances are assigned in a sequential manner. In addition, some of the H3’ sugar proton resonances are also assigned and some of the exchangeable amino proton resonances identified. The relative magnitudes of the intranucleotide and internucleotide NOES are indicative of a right-handed B-type conformation for the duplex undecamer in solution.
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